anti-cd62l monoclonal antibody mel-14  (Bio X Cell)

Journal: iScience

Article Title: AF6 regulates intestinal IgA via crosstalk between intestinal epithelial cells and immune cells in inflammatory bowel disease

doi: 10.1016/j.isci.2025.112658

Figure Lengend Snippet: AF6 deficiency in IECs impairs B cell signaling and IgA production in mice with colitis (A) GO pathway enrichment analysis of colonic tissue transcriptomes in Af6 f/f and Af6 ΔIEC mice maintained on 2.5% DSS for 7 days. (B) GSEA analysis, based on GO, of colonic tissue transcriptomes in Af6 f/f and Af6 ΔIEC mice maintained on 2.5% DSS for 7 days. NES, standardized enrichment scores. (C) Flow cytometric analysis was used to measure the proportion of IgA + B220 - cells in the colon lamina propria of Af6 f/f and Af6 ΔIEC mice maintained on 2.5% DSS for 7 days. (D and E) (D) IgA content was measured by ELISA in feces (after 3 days on DSS-containing drinking water) and serum (after 7 days on DSS-containing drinking water) of Af6 f/f and Af6 ΔIEC mice. Flow cytometric analysis was used to measure the proportion of (E) MHCII + CD11c + cells and (F) CD44 + CD62L − in the lamina propria of Af6 f/f and Af6 ΔIEC mice maintained on 2.5% DSS for 7 days, n = 5–8 mice per group. Data are expressed as mean ± SEM. Pairwise comparisons between groups were conducted using two-tailed non-paired Student’s t tests. p -values were shown in the panel, and p < 0.05 indicates a significant difference.

Article Snippet: CD62L (L-Selectin) Monoclonal Antibody (MEL-14), eFluorTM 450 , eBioscience , Cat#48-0621-82.

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: eLife

Article Title: PIM kinase control of CD8 T cell protein synthesis and cell trafficking

doi: 10.7554/eLife.98622

Figure Lengend Snippet: ( A ) Estimated copies per cell of PIM1 and PIM2 protein from published quantitative proteomics analysis ; of CD8 T cells expanded in IL-2 or IL-15 as outlined in . ( B–D, F, G ) WT (Ly5.1) and Pim dKO lymph node or spleen single-cell suspensions were mixed at a 50:50 ratio of T cells, activated for 2 days with αCD3/αCD28 (both 0.5 µg/mL) and IL-2 (20 ng/mL), washed then split into fresh medium containing IL-2 (20 ng/mL) daily (as per ). Some of the mixed cell suspensions were also cultured in IL-7 (5 ng/mL) to sustain a naive T cell reference. ( B ) WT and Pim dKO CTL were treated 1 hr +/- Jak1/3 inhibitor Tofacitinib (100 nM; negative control) before pSTAT5 Y694 expression was measured on day 3 and 6 of culture, ( C ) surface CD25 expression was measured on days 3 and 6 of culture, ( D ) CD8 T cell number vs time was calculated, ( F ) CD8 T cell FSC-A, SSC-A and surface activation markers (CD44, CD71) were measured on days 3 and 6 of culture ( G ) expression of adhesion molecule CD62L was measured daily. ( E ) WT and Pim dKO T cells were activated and expanded with IL-2 as per ( B-D ) and ( F, G ) except in separate cultures and % live cells (PI-ve) was assessed on days 4 and 6 (two-way ANOVA). Symbols in bar charts represent biological replicates, symbols in ( D ) represent the mean. Error bars show mean ± S.D. Data are representative of ( B, G ) n=4, ( C, F ) n=6 or show pooled data from ( D ) n=4, ( E ) n=6 biological replicates with data collected over at least two independent experiments. Figure 3—source data 1. Raw values plotted in .

Article Snippet: Antibody , anti-mouse CD62L (Rat, monoclonal, MEL-14) , Thermofisher Scientific/eBioscience , Cat # 12-0621-83, RRID: AB_465722 , Cell surface stain 1:200, PE.

Techniques: Quantitative Proteomics, Cell Culture, Negative Control, Expressing, Activation Assay

Journal: Journal of Lipid Research

Article Title: A single-day mouse mesenteric lymph surgery in mice: an updated approach to study dietary lipid absorption, chylomicron secretion, and lymphocyte dynamics

doi: 10.1016/j.jlr.2022.100284

Figure Lengend Snippet: Reagents used for flow cytometry analysis

Article Snippet: CD62L (L-Selectin) Monoclonal Antibody (MEL-14), APC (1:400 diluion) , 17-0621-82 , Thermo Fisher Scientific.

Techniques: Flow Cytometry, Staining

Journal: iScience

Article Title: Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes

doi: 10.1016/j.isci.2022.104998

Figure Lengend Snippet: Frequency and phenotype of ST2 + Tregs across tissues The frequency and phenotype of Tregs from Foxp3 YFP−cre mice were assessed by flow cytometry. (A) Representative flow plots showing ST2 vs CD4, gated on live singlets, CD45 + , TCRβ + CD4 + FOXP3 + cells in blood, spleen, lungs, VAT, colon, and skin. (B) Frequency of ST2-expressing Tregs across tissues, based on gating in Figure 1 A. Data are pooled from 7 experiments, each with n = 4–6 per cohort. (C) Representative overlays of ST2 + Tregs (red) on total Tregs (gray), showing CD62L vs CD44 expression in indicated tissues, pregated on live singlets, CD45 + , TCRβ + CD4 + FOXP3 + cells. (D–F) Left: representative flow plots and histograms showing staining of (D) KLRG1, (E) CD103, and (F) Ki-67 in selected tissues and populations (orange: ST2 + Tregs, blue: ST2 - Tregs, red: Foxp3 - CD4 + T cells), Right: frequency of (D) KLRG1, (E) CD103, and (F) Ki-67 on ST2 + Tregs, ST2 - Tregs, and Foxp3 - CD4 + T cells across tissues. D and F: Data are pooled from 3 experiments of n = 5 each. E: Data are pooled from 2 experiments of n = 5 each, except for VAT and colon with n = 4 in one of 2 experiments. Data are plotted as mean ± SD and indicated means were compared with two-way ANOVA with Sidak’s multiple comparisons test, ns p > 0.05, ∗∗∗∗p < 0.0001. Abbreviations: VAT: visceral adipose tissue, TCR: T cell receptor. See Figure S1 for flow cytometric gating and analysis of Tregs across tissues and in secondary lymphoid organs.

Article Snippet: APC-eFlour780 CD62L Monoclonal Antibody Clone MEL-14 , eBioscience , Cat# 47-0621-82; RRID: AB_1603256.

Techniques: Flow Cytometry, Expressing, Staining

Journal: iScience

Article Title: Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes

doi: 10.1016/j.isci.2022.104998

Figure Lengend Snippet: RNAseq analysis of ST2 + Tregs and ST2 - Tregs across tissues RNAseq was performed on flow sorted ST2 + CD44 hi Tregs and ST2 - CD44 hi Tregs from 6 different tissue sites (blood, spleen, lungs, VAT, colon, and back skin) and ST2 - CD44 lo CD62L + Tregs from spleen and lungs. Data are from 5 male Foxp3 YFP−cre mice ages 16–19 weeks old. (A) PCA based on all genes from all populations and tissues. The percentages alongside the PC axes labels are the percentage of variance explained by each PC. (B) Dendrogram and correlation coefficient using Spearman’s ranked-based correlation analysis of all genes in libraries from ST2 + CD44 hi Tregs and ST2 - CD44 hi Tregs. (C) Heatmap of pooled tissue-specific DEGs, sorted by tissue. Data displayed as Z score. Abbreviations: VAT: visceral adipose tissue, PCA: principal component analysis, DEG: differentially expressed genes. See Figure S3 for the correlation of factors/categorical variables across PC coordinates and Venn diagrams identifying tissue-specific DEGs displayed in Figure 3 C.

Article Snippet: APC-eFlour780 CD62L Monoclonal Antibody Clone MEL-14 , eBioscience , Cat# 47-0621-82; RRID: AB_1603256.

Techniques:

Journal: iScience

Article Title: Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes

doi: 10.1016/j.isci.2022.104998

Figure Lengend Snippet:

Article Snippet: APC-eFlour780 CD62L Monoclonal Antibody Clone MEL-14 , eBioscience , Cat# 47-0621-82; RRID: AB_1603256.

Techniques: Recombinant, Staining, Cell Isolation, Sequencing, Sample Prep, Software

Journal: iScience

Article Title: Nicotinamide breaks effector CD8 T cell responses by targeting mTOR signaling

doi: 10.1016/j.isci.2022.103932

Figure Lengend Snippet:

Article Snippet: Rat monoclonal α-CD62L (MEL-14) , Thermo Fisher Scientific , Cat# 12-0621-82; RRID: AB_465721.

Techniques: Recombinant, Staining, Activation Assay, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Software